polynucleotide phosphorylase

Studies on polynucleotide phosphorylase of Escherichia coli ⅰ . purification and properties

大肠杆菌多核苷酸磷酸化酶的研究Ⅰ.酶的提纯与性质

OBJECTIVE : Selection of optimal chromatographic conditions for purification of polynucleotide phosphorylase in fast ion-exchange chromatography .

目的：选择多核苷酸磷酸化酶快速离子交换层析的最佳条件。

Preparation of Poly I and poly C through polymerization catalyzed by immobilized polynucleotide phosphorylase

固定化多聚核苷酸磷酸化酶聚合生产polyⅠ和polyC的研究

Studies on immobilized polynucleotide phosphorylase ⅱ . the application of immobilized enzyme for the preparation of Poly I and poly C

固定化多核苷酸磷酸化酶的研究&Ⅱ.用固定化酶制备PolyI和PolyC

Its principle is based on the polymerization of 7-methylguanosine 5 ' diphosphate ( m ~ 7GDP ) on an oligonucleotide primer in the presence of polynucleotide phosphorylase ( PNPase ), followed by selective elimination of m ~ 7G under mild conditions .

其原理是用多核苷酸磷酸化酶（PNPase），使7-甲基鸟苷5'二磷酸（m~7GDP）在引物存在下聚合，然后在温和的化学条件下，选择性消去7-甲基鸟苷（m~7G）。